Piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES) CAS 5625-37-6
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Product Description
Product Details
Introduction
PIPES cannot form stable complexes with most metal ions and is suitable as a buffer in solution systems containing metal ions. It is used to purify tubulin using phosphocellulose chromatography, to purify recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and as a buffer to crystallize transketolase from Escherichia coli. In addition, since PIPES can form free radicals, it is not suitable for use in redox systems. In cation exchange chromatography, a low concentration of PIPES buffer should be used because PIPES has a relatively large ionic strength and its pKa value is concentration-dependent.
Specification
Appearance | White powder |
Content | ≥99% |
PH | 6.1-7.5 |
pKa (25 °C) | 6.8 |
Application
Biological buffers are used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits.
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