Nitrotetrazolium blue chloride (NBT) CAS 298-83-9
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Product Description
Product Details
Introduction
Nitrotetrazolium blue chloride (NBT) is a tetrazole compound with two tetrazole rings. It is often mixed with bcip and is one of the substrates of alkaline phosphatase. It generates an insoluble blue product under the catalysis of alkaline phosphatase. It is often used in experiments such as immunohistochemistry (IHC), immunocytochemistry (ICC) and western blotting (WB).
Physical indicators
| Appearance | Light yellow powder |
| Purity | ≥98.0% |
| Solubility | H2O: 10 mg/mL; soluble in most organic solvents such as methanol and ethanol, slightly soluble in ethyl acetate, insoluble in ether. |
| Melting point | 200°C |
| Ignition residue | <0.5% |
| Moisture | <4.5% |
Application
1、It is one of the substrates of alkaline phosphatase, and generates an insoluble blue product under the catalysis of alkaline phosphatase. It is commonly used in experiments such as immunohistochemistry (IHC), immunocytochemistry (ICC) and western blot (WB).
2、It is a substrate for dehydrogenases and other peroxidases. It is used to determine glucose-6-phosphate dehydrogenase; it is also a precipitation reagent for coagulation factor I (fibrinogen).
3、NBT can be used as a method to screen for urinary tract infections. The nitro blue tetrazolium chloride (NBT) reduction method is used to determine serum glycosylated low-density lipoprotein and very low-density lipoprotein (SG LP-V P). The concentration of SG LP-V P is directly expressed by optical density according to the depth of color, and the index of SG LP-V P is calculated, and the results are very good.
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