NSP-DMAE- NHS ester CAS 194357-64-7

Introduction

Acridinium esters are a class of chemical substances that can be used as chemiluminescent markers. In alkaline H₂O₂ solution, when the molecules of acridinium esters are attacked by hydrogen peroxide ions, the substituents on the acridinium ring can react with C-9 on the acridinium ring and H₂O₂ (hydrogen peroxide) to form unstable ethylene dioxide, which decomposes into CO₂ and electronically excited N-methylacridone.

It can be used for research such as chemiluminescence and immunoassay, receptor analysis, and nucleic acid and peptide detection.

Applications:

1. Direct labeling of antigens and antibodies;

2. Detection of thyroid levels;

3. Detection of marker alpha-fetoprotein;

4. Analysis of immunoglobulins and related compounds, etc.

Advantages:

1. NSP-DMAE-NHS is simple to luminesce, does not require a catalyst, can reduce background luminescence, improve sensitivity and reduce interference.

2. NSP-DMAE-NHS has high luminescence efficiency and high intensity because the luminescence speed is fast; usually, the entire luminescence process can be completed in 1-2 seconds, which is five times or more than luminol.

3. NSP-DMAE-NHS has few luminescence interference factors, low background, and a high signal-to-noise ratio, and its luminescence efficiency is basically not affected by the structure of the substituent.

4. NSP-DMAE-NHS has a small molecular weight and is easy to be firmly connected to the protein through chemical bonds. It has little effect on the conformation of the antibody after binding.

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